Fig 6. Phenotypic effects of depletion of MuvB or E2F-DP in lin-35 null worms.

(A,B) Strip chart of the brood size of (A) lin-35 null worms following efl-1, lin-9, or lin-54 RNAi at 24°C and (B) wild-type (WT), single DRM mutants, and double DRM mutants at 20°C. Each dot indicates the total brood of an individual worm, and a red bar indicates the median for each genotype. Significance (** p-value < 0.01) was determined by a Wilcoxon-Mann-Whitney test comparing RNAi to empty vector control (Ctrl, A) or double mutants to their respective single mutants (B). (C) DAPI staining of young adults. The filled arrowhead indicates a representative healthy oocyte, open arrowheads indicate representative endomitotic oocytes (EMO), and asterisks indicate normal-appearing embryos. No EMO: no DAPI-stained DNA masses in the oviduct, and presence of normal-appearing embryos in the uterus. Moderate EMO: presence of some DAPI-stained DNA masses in the oviduct and some normal-appearing embryos in the uterus. Severe EMO: presence of DAPI-stained DNA masses in the oviduct and no normal-appearing embryos in the uterus. Scale bar, 50 μm. (D) Normalized stacked bar chart of the EMO phenotype of Day 1 adult lin-35 null worms with DRM subunit RNAi or double DRM mutants compared to single mutants and WT.