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. 2017 Oct 20;9:58–68. doi: 10.1016/j.plabm.2017.10.003

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Western blot analyses showing detection of monomeric (approximately 105 kDa) and dimeric dipeptidyl peptidase-4 (DPP-4) forms (approximately 210 kDa) by detection (A) and capture (B) antibodies, respectively, used in the ARCHITECT DPP-4 Immunoassay. Lane 1: Dual Xtra Molecular Weight Markers; Lane 2: Empty; Lane 3: DPP-4 fragment (reduced and boiled); Lane 4: DPP-4 fragment (reduced and non-boiled); Lane 5: DPP-4 fragment (non-reduced and boiled); Lane 6: DPP-4 fragment (non-reduced and non-boiled). Abbreviation: DPP-4: dipeptidyl peptidase-4.