Table 2. Mycobacterial and Cytotoxicity Profiles of Indolylpropyl-TPP (8d, 11d) and Indolylpentyl-TPP (9a, 12a) Analogues.
|
M.
tuberculosis H37Rva (μM) |
bactericidal
activity M. bovis BCG (μM)d |
|||||
|---|---|---|---|---|---|---|
| no. | MIC50 | MIC90 | Vero IC50b (μM) | RBC HC50c(μM) | MBC99 | MBC99.9 |
| 8d | 6 | 20 | 34 | >300 | 13 | 25 |
| 11d | 2 | 6 | 18 | >300 | 3 | 6 |
| 9a | 22 | 45 | >100 | 230 | 25 | 50 |
| 12a | 4 | 6 | 36 | 196 | 3 | 6 |
a
Minimum inhibitory concentrations required to reduce bacterial growth by 50% (MIC50) and 90% (MIC90) compared to untreated controls.
b
Concentration required to inhibit mammalian Vero cell viability by 50%, compared to untreated control cells.
c
Concentration required to lyse 50% of human RBC compared to positive control Triton-X 100 (2% v/v) which induced total hemolysis (100%).
d
Minimum bactericidal concentrations required to reduce initial number of colony forming units (CFU) by 99% (MBC99) and 99.9% (MBC99.9) compared to untreated controls. Average of two or more separate determinations.