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A: Thy-1(−) cells, were transfected with Thy-1 and then sorted and retested for Thy-1 expression by FACS. Thy-1 (+) and (−) sorted cells were plated and stimulated with TGF-β for 24 h. B: Gel zymography showing lack of MMP9 band activity in the cells in which Thy-1 is re-expressed. The non-transfected cells show MMP-9 activity under TGF-β stimulation.
