Figure 4.

Impaired Heart Function Due to Cardiac ATGL Deficiency Causes Hypothermia

(A and D) Kaplan-Meier plots depicting mice maintaining euthermia (body temperature ≥35°C) during acute cold exposure at 5°C at indicated time points from AKO (A) and iHAKO mice (D).

(B, E, and H) Left ventricular (LV) mass from AKO (B), iHAKO (E), and AKO/cTg mice (H).

(C, F, and I) Ejection fraction from AKO (C), iHAKO (F), and AKO/cTg mice (I).

(G) Body temperature of male AKO/cTg mice during acute cold at 5°C for 3–6 hr (n ≥ 8).

Male AKO mice were analyzed at the age of 6 and 10 weeks (n ≥ 6). iHAKO mice of mixed sex were studied at the age of 12 months and 6 and 10 weeks upon tamoxifen administration (postTAM) (n ≥ 6). Male AKO/cTg mice aged 5–6 months were used to study LV mass and ejection fraction (n = 5). Data are presented as means ± SD, except for Kaplan-Meier plot showing median. Statistical significance was evaluated by Gehan-Breslow-Wilcoxon test for Kaplan-Meier plots or two-way ANOVA and Bonferroni post hoc tests. ^∗∗p < 0.01 and ^∗∗∗p < 0.001 versus control and ^###p < 0.001 versus 6-week-old respective groups. See also Figures S4 and S5.