REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ATGL	Cell Signaling	Cat#2138; RRID: AB_2167955
UCP-1	Abcam	Cat#ab10983; RRID: AB_2241462
Glycerol kinase (GyK)	Abcam	Cat#ab126599; RRID: AB_11129767
GAPDH	Cell Signaling	Cat#2118; RRID: AB_561053
Ndufs1	Abcam	Cat#ab157221
Rabbit-HRP	Cell Signaling	Cat#7074; RRID: AB_2099233
Chemicals, Peptides, and Recombinant Proteins
CL 316,243	Sigma	Cat#C5976
Leupeptin	Roth	Cat#CN33.3
Antipain	Roth	Cat#2933.2
Pepstatin	Roth	Cat#2936.2
PVDF membrane, 0.45 μm	Roth	Cat#T830.1
Protein Assay Dye	Bio-Rad	Cat#500-0006
BCA Protein Assay	Pierce	Cat#23225
Clarity Western ECL Substrate	Bio-Rad	Cat#170-5061
Triolein	Sigma-Aldrich	Cat#T7140
Triolein, [9,10-3H(N)]	Perkin Elmer	Cat#NET431L
Phosphatidyl-Choline	Sigma-Aldrich	Cat#P3556
Phosphatidyl-Inositiol	Sigma-Aldrich	Cat#P0639
BSA, essentially fatty acid free, 96%	Sigma-Aldrich	Cat#A6003
Rotiszint eco plus (scintillation cocktail)	Roth	Cat#0016.3
Collagenase Type CLS II	Worthington	Cat#LS004177
Dispase II	Sigma-Aldrich	Cat#04942078001
DNeasy Blood & Tissue Kit	QIAGEN	Cat#69504
Trizol	Invitrogen, Thermo Fisher Scientific	Cat#15596-018
Dnase I	Invitrogen, Thermo Fisher Scientific	Cat#18068-015
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems, Thermo Fisher Scientific	Cat#4374966
Maxima SYBR Green	Fermentas, Thermo Fisher Scientific	Cat#K0223
Pyruvate, sodium salt	Sigma-Aldrich	Cat#P2256
Glycerol-3-phosphate, bis(cyclohexylammonium) salt	Sigma-Aldrich	Cat#G7886
Rotenone	Sigma-Aldrich	Cat#R8875
Guanosine 5′-diphosphate, disodium salt	Sigma-Aldrich	Cat#51060
Atglistatin (ATGLi)	Mayer et al., 2013	N/A
HSL inhibitor (HSLi)	Novo Nordisk, DK; Schweiger et al., 2006	N/A
Critical Commercial Assays
NEFA-HR(2) R1 Set	Wako Chemicals	Cat#434-91795
NEFA-HR(2) R2 Set	Wako Chemicals	Cat#436-91995
Infinity Triglyceride Reagent	Thermo Fisher Scientific	Cat#TR22421
Free gylcerol reagent	Sigma-Aldrich	Cat#F6428
Experimental Models: Organisms/Strains
Mouse: AKO: ATGL−/−; B6;129P2-Pnpla2tm1Rze/J	(Haemmerle et al., 2006); The Jackson Laboratory	Cat#019003
Mouse: AKO/cTg: ATGL−/−/α-MHC-ATGL transgene+/−	(Haemmerle et al., 2011, Schreiber et al., 2015)	N/A
Mouse: ATGLflox/flox: B6N.129S-Pnpla2tm1Eek/J	(Sitnick et al., 2013); The Jackson Laboratory	Cat#024278
Mouse: UCP1-CreER	(Rosenwald et al., 2013)	N/A
Mouse: Adiponectin-Cre: B6; FVB-Tg(Adipoq-cre)1Evdr/J	(Eguchi et al., 2011); The Jackson Laboratory	Cat#010803
Mouse: α-MHC-MerCreMer: B6.FVB(129)-A1cfTg(Myh6-cre/Esr1∗)1Jmk/J	The Jackson Laboratory	Cat#005657
Mouse: iBAKO: ATGLflox/flox/ UCP1-CreER+/−	This paper	N/A
Mouse: AAKO: ATGLflox/flox/ Adiponectin-Cre+/−	(Schoiswohl et al., 2015); this paper	N/A
Mouse: iHAKO: ATGLflox/flox/ α-MHC-MerCreMer+/−	(Kienesberger et al., 2013); this paper	N/A
Mouse: UKO: UCP1−/−; B6.129-Ucp1tm1Kz/J	The Jackson Laboratory	Cat#003124
Oligonucleotides
A full list of sequences is listed in Table S3.	This paper	N/A
Software and Algorithms
Image Lab software (version 5.2.1)	Bio-Rad	http://www.bio-rad.com/en-us/product/image-lab-software
PhenoMaster software	TSE	N/A
Dataquest A.R.T. software (version 4.31)	DSI	N/A
DatLab software (version 5.1.1.91)	OROBOROS Instruments	N/A
MATLAB	MathWorks	N/A
itk-SNAP (version 3.2.0)	(Yushkevich et al., 2006)	http://www.itksnap.org/pmwiki/pmwiki.php
GraphPad Prism 6	GraphPad Software	N/A
SPSS Statistics 24	IBM	N/A
Other
R/M-H Extrudate	Ssniff	Cat#V1126-037