Table 2.
Additions, μM |
45Ca influx, pmol/mg protein/5 min |
|
---|---|---|
[K+]o=5 mM | [K+]o=30 mM | |
1. None (control) | 100 | 278±23* |
2. NaHS, 100 | 110±20 | 387±25* |
3. NaHS, 100 + bumetanide, 10 | 101±17 | 289±22*, # |
4. NaHS, 100 + TEA, 104 | 108±19 | 367±30* |
5. NaHS, 100 + nicardipine, 2 | 125±21 | 104±10# |
Means±S.E. obtained in 5 independent experiments performed in quadruplicates are shown. The rate of Ca2+ influx at [K+]o = 5 mM and in the absence of any additions listed in the left column varied from 389 and 425 nmol / mg protein / 5 min was taken as 100%.
- p < 0.05 compared to 45Ca influx at [K+]o = 5 mM.
- p<0.05 compared to control at [K+]o = 30 mM in the presence of 100 μM NaHS ( there was no significant differences of Ca2+ influx in the presence of additions at [K+]o = 5 mM).