Table 3.
Additions, μM |
45Ca influx, pmol/mg protein/5 min |
|
---|---|---|
[K+]o=5 mM | [K+]o=30 mM | |
1. None (control) | 100 | 278±23* |
2. NaHS, 1000 | 106±15 | 177±18* |
3. NaHS, 1000 + bumetanide, 10 | 113±21 | 170±25* |
4. NaHS, 1000 + TEA, 104 | 112±16 | 277±18*, # |
5. NaHS, 1000 + nicardipine, 2 | 88±14 | 100±12# |
Means ± S.E. obtained in 5 independent experiments performed in quadruplicates are shown. The rate of Ca2+ influx at [K+]o = 5 mM and in the absence of any additions listed in the left column varied from 389 and 425 nmol / mg protein / 5 min was taken as 100%.
- p < 0.05 compared to 45Ca influx at [K+]o = 5 mM.
p<0.05 compared to control at [K+]o = 30 mM, in the presence of 1000 μM NaHS ( there was no significant differences of Ca2+ influx in the presence of additions at [K+]o = 5 mM).