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. Author manuscript; available in PMC: 2017 Nov 13.
Published in final edited form as: Pediatr Res. 2016 Jul 18;80(5):719–728. doi: 10.1038/pr.2016.143

Figure 4.

Figure 4

Quantification of cleaved caspase-3 and PCNA protein abundance in preterm lambs supported by NIS, with or without excess sedation (ES). White fill indicates NIS control, hatched fill indicates NIS+ES. Results are shown as mean ± SD for n=4/group. Panel a: Cleaved caspase-3 protein abundance. Panel b: PCNA protein abundance. Cleaved caspase-3 abundance is significantly lower in lungs of NIS+ES group compared to NIS control group (*different from the NIS group by Mann-Whitney U test, p < 0.05). Panels c and d: NIS immunohistochemistry of (c) cleaved caspase 3 and (d) PCNA. Panels e and f: NIS+ES immunohistochemistry of (e) cleaved caspase 3 and (f) PCNA. Immunohistochemistry showed that fewer mesenchymal-cell nuclei were immunostained brown for cleaved caspase 3 protein. The immunostain pattern for PCNA protein was similar (scale bar is 100 μm). Insets are enlargements of the region identified by the arrow in each panel.