Figure 1.

Intracranial infection with Theiler’s murine encephalomyelitis virus (TMEV)-OVA8 generates long lived T_RM. (A) Central nervous system (CNS) infiltrating lymphocytes from intraperitoneally or intracranially infected C57BL/6 mice were analyzed 140 days post-infection for antigen specific CD8⁺ T-cell responses using the virus specific tetramer H-2K^b-OVA8 or the non-specific control tetramer H-2K^b-SIYR (n = 3 for each group). (B) Splenocytes from C57BL/6 infected mice infected with TMEV-OVA8 were analyzed with the virus specific tetramer H-2D^b-VP2_121–130 to determine the frequency of virus specific CD8⁺ T-cells. (C) Analysis of CD8⁺ cells recovered from the CNS and spleens of C57BL/6 mice for CD44 expression on 6 day 6 and 98 post infection with TMEV-OVA8. Numbers are mean fluorescence intensity (MFI; mean + SD, n = 5 per time point). (D) CD69 and CD103 expression on H-2K^b-OVA8⁺ CD8⁺ T-cells isolated from the CNS of C57BL/6 mice intracranially infected with TMEV-OVA8 on day 140 post-infection (n = 3 for each group). Data are expressed as mean + SD.