Figure 3.

The accumulation of viral antigen specific CD8⁺ T-cells in the CNS of B7-H1^KO mice post Theiler’s murine encephalomyelitis virus (TMEV) infection. (A) CD45⁺ cells recovered from the CNS were analyzed for percent of total OVA8⁺ CD8⁺ T-cells in B7-H1^WT and B7-H1^KO mice on day 6 and day 98 post intracranial TMEV-OVA8 infection (n = 5 per group; mean + SD percent of total CD8⁺ cells). (B) CD45⁺ gated cells in (A) were analyzed to determine the percent of B7-H1^WT and B7-H1^KO CD8⁺ T-cells that were specific to the TMEV peptide antigen VP2_121–130 on day 6 and day 98. (C) Absolute numbers of total CD8⁺ tetramer positive OVA8⁺ and VP2⁺ CD8⁺ T-cells recovered from the CNS of B7-H1^WT and B7-H1^KO mice on day 6 and day 98 post TMEV-OVA8 intracranial challenge. *denotes significance of p < 0.05 by t-test when comparing B7-H1^WT and B7-H1^KO.