Figure 3.

CpG induces NLRP3-mediated antibody production. IgM (A,B), IL-6, TNF-α, and MMP-7 (C) were measured by ELISA in the cell supernatant of unstimulated (NTC), CpG, AF1 and AF2 stimulated cells, and CpG stimulated cells in the presence of different concentrations of MCC950 as indicated. Cells were left untreated (NTC) or pre-incubated with 50 mM KCL, 50 µM oxidized ATP (oxATP) (D), and the caspase-1 inhibitors, YVAD-CHO and YVAD-CMK, at a concentration of 50 µM each (E) prior to stimulation with CpG as indicated. 1 µg/ml CpG was used unless otherwise specified. Data are representative of at least three donors in each independent experiments (n = 3). ***p < 0.0001.