Figure 4.

Dectin-1 partially mediates IL-1β secretion and requires SYK and the transcriptions factors AP-1 and NF-κB. IL-1β ELISA measured in the cell supernatant of curdlan (Curd) stimulated cells in the presence or absence of 1 mg/ml Laminarin (Lamin) (A), 5 µg/ml anti-dectin-1 antibody (α-Dect1) or 5 µg/ml isotype control (Ms IgG) (B), 40 µM of the SYK inhibitor, piceatannol (pice) and 5 µM of the SYK inhibitor, R406 (C), and 10 µM of the NF-κB inhibitor, Bay11, and 10 µM of the AP-1 inhibitor, SR11 (D). Data are representative of at least three donors in each independent experiments (n = 3). *p < 0.05, ***p < 0.0001.