FIGURE 2.

CHPG treatment increases purinergic current through P2X7 receptors in basal but not LPS-activated conditions. In (A), representative traces of purinergic current recorded in BV2 cells in the following conditions: basal (C), after 24 h treatment with CHPG 200 μM, after 6 h treatment with LPS 0.1 μg/ml and after co-treatment of CHPG for 24 h and LPS added during the last 6 h. Currents were elicited after stimulation of P2X7 receptors with the selective agonist Bz-ATP, 100 μM. Summary of purinergic current elicited from cells stimulated as in (A) are reported in (B). Data are presented as current (pA) obtained by each cell and normalized to membrane capacitance (Cm), an estimation of cellular surface area. Bars are mean ± SEM (n = 11 for C; n = 8 for CHPG; n = 9 for LPS; and LPS + CHPG), ^∗p < 0.05 by one-way ANOVA followed by Newman–Keuls post hoc test. Summary of Cm measured for each cell in different conditions (C). Bars are mean ± SEM of corresponding cells used for bar diagram in (B).