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. 2017 Nov 9;8:812. doi: 10.3389/fphar.2017.00812

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Copyright © 2017 Beneventano, Spampinato, Merlo, Chisari, Platania, Ragusa, Purrello, Nicoletti and Sortino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CHPG increases miRNA146a levels in BV2 cells-shed MVs. BV2 cells were activated with LPS (0.1 μg/ml for 6 h) after pretreatment with CHPG (200 μM, total exposure 24 h). MVs were isolated, miRNA extracted and levels of miRNA146a were analyzed by real time PCR (A). In (B), levels of miRNA146a were analyzed in MVs derived from MPTEP pretreated-cells (100 μM added 30 min before CHPG). Results are expressed as percentage of CHPG. One-way ANOVA followed by Newman–Keuls test (A) and Student’s t-test (B) were applied for significance. ^∗p < 0.05 vs. control (A); ^∗p < 0.05 vs. CHPG (B).