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. 2017 Nov 9;8:1498. doi: 10.3389/fimmu.2017.01498

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Copyright © 2017 Hellebrekers, Hietbrink, Vrisekoop, Leenen and Koenderman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of randomness in the interaction between Staphylococcus aureus- green fluorescent protein (GFP) and neutrophils. The experiment was performed with a MOI of 1 S. aureus-GFP in the first round (A) and with -mCherry in the second round (B). A representative experiment is shown. Ratios in the mCherry positive (/+) and negative cells (/−) were determined in the GFP-positive (+/) and negative (−/) (C) and MOI 1 and 2 (D) cells. (C) A relative enrichment of +/+ and −/− neutrophils is seen compared to the +/− and −/+ cells, which is not to be expected in a random process (***P = 0.0005). Differently colored dots represents results from the same experiments, different experiments can be executed with different MOIs. (D) An increase in mean fluorescence intensity can be observed in the mCherry positive group of the MOI 2 condition compared to a MOI 1 (ns, P = 0.125). Data were analyzed with the Wilcoxon test.