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. 2017 Nov 9;8:1498. doi: 10.3389/fimmu.2017.01498

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Copyright © 2017 Hellebrekers, Hietbrink, Vrisekoop, Leenen and Koenderman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis to test the feed-forward hypothesis. Neutrophils were first mixed with Staphylococcus aureus -Cherry with an MOI of 1 and a clear distribution is seen into positive and negative cells (A). (B) The same experiment was performed in cells from the same donor preincubated with S. aureus-GFP at an MOI of 10. All neutrophils were characterized in the first round by 100% phagocytosis all neutrophils become GFP-positive[panel (B)]. Adding S. aureus-mCherry with a MOI of 1 to these GFP-positive neutrophils resulted the same distribution pattern that was observed before adding S. aureus-GFP [see panel (A)]. Depicted are representative samples for three independent experiments.