Table 1.

Phagocytosis assays to assess randomness and feed-forward mechansims.

First round	Second round	Aim
No bacteria	MOI 1/2	Serve as a control for phagocytizing capability after 40 min at 37°C
No bacteria	MOI 10
MOI 1	MOI 1/2	Observe and compare distributions of first and second round bacteria
MOI 1	MOI 10
MOI 10	MOI 1/2	Observe the effect of activation of all neutrophils on second round phagocytosis
MOI 10	MOI 10

Staphylococcus aureus-green fluorescent protein was mostly used in the first round and -mCherry in the second.