Table 1.
First round | Second round | Aim |
---|---|---|
No bacteria | MOI 1/2 | Serve as a control for phagocytizing capability after 40 min at 37°C |
No bacteria | MOI 10 | |
MOI 1 | MOI 1/2 | Observe and compare distributions of first and second round bacteria |
MOI 1 | MOI 10 | |
MOI 10 | MOI 1/2 | Observe the effect of activation of all neutrophils on second round phagocytosis |
MOI 10 | MOI 10 |
Staphylococcus aureus-green fluorescent protein was mostly used in the first round and -mCherry in the second.