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. 2017 Nov 13;7:15407. doi: 10.1038/s41598-017-15676-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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JMJD8 forms dimers or oligomers. (a) HEK293T cells were transfected with plasmids that express the indicated proteins. The interaction between wild-type and mutant JMJD8 was determined with an immunoprecipitation assay using an anti-HA antibody or mouse IgG. Total cell lysates and immunoprecipitated products were immunoblotted with the indicated antibodies (n = 3). (b) Total cell lysate from HeLa S3 cells stably expressing JMJD8-FLAG-HA (HeLa-JMJD8-FLAG-HA) was subjected to gel filtration (Superdex 200) analysis. The fractions were collected and immunoblotted with an anti-FLAG antibody. Full-length blots are presented in Supplementary Fig. S6.