Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 13;7:15432. doi: 10.1038/s41598-017-15648-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Maps of donor vectors and analysis of specific integration in transfected clones. (A) pDB2 vector with CpG-free EF1α promoter. (B) pDB2 vector with EF1α promoter. (C) pDB2 vector with CMV promoter. The maps were designed by SnapGene 1.1.3 software. (D) EGFP coding sequence was amplified in different isolated clones by genomic PCR using EGFPF and EGFPR primers. In lanes 1–8, 714-bp bands confirmed the general integration of the donor vector in recombinant clones. (E) The second genomic PCR was designed to confirm the specificity of genomic integration using attBF and attBR primers. The lack of 290-bp band in recombinant clones (lanes 1, 2, 4, 7 and 8) established that phiC31 catalyzed the targeted integration between attB and pseudo-attP sites. M is DNA ladder 100 bp. Ctrl(+) is positive control in which PCR reaction was performed on pDB2 plasmid and Ctrl(−) is negative control in which PCR reaction was carried out on the genome of non-transfected cells. Electrophoretic gel images presented in this figure were full length.