Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 13;7:15432. doi: 10.1038/s41598-017-15648-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Rescue and analysis of integration site for donor vectors. (A) Recombination between attB and BF10 pseudo-attP sites in the cattle genome was verified using semi-nested PCR. The products were amplified using primers attBF3, 885R and BF10nested. Lanes 1–4, 7, 8, 10 and 11 show the integration of donor vector into BF10 site. (B) Recombination between attB and BF10 sites was also verified by nested PCR using attBF3 and BF10 nested primers. Lanes 2, 6, 8 and 10 show the integration of donor vector into BF10 site. (C) Recombination between attB and BF4 pseudo-attP sites was verified by semi-nested PCR using attR, attR928L and BF4nested primers. Lanes 1–6 show the vector integration into BF4 site. (D) Recombination between attB and BF4 sites was also verified by nested PCR using attR and BF4 primers. Lanes 2–10 show the successful integration into BF4 site. M is DNA ladder 100 bp. Electrophoretic gel images presented in this figure were full length.