Table 3.
Analysis of in vitro blastocyst development after SCNT using single-copy clones.
| Donor cell | Promoter | Insertion site | No. culture | *No cleaved (%) | **No blastocyst (%) |
|---|---|---|---|---|---|
| C1.1 | CMV | BF4 | 161 | (85% ± 0.10) | (7.0% ± 0.50) |
| C1.2 | CMV | BF10 | 144 | (83% ± 5.50) | (8.8% ± 2.40) |
| C.1.3 | CMV | BF5 | 146 | (86% ± 1.00) | (15.4% ± 0.65) |
| E1.1 | EF1α | BF4 | 180 | (91% ± 4.00) | (8.3% ± 0.55) |
| E1.2 | EF1α | BF5 | 184 | (87% ± 1.00) | (18% ± 0.10) |
| E1.3 | EF1α | BF10 | 166 | (83% ± 4.00) | (8.8% ± 0.45) |
| CE.1.1 | CpG-free EF1α | BF4 | 118 | (95% ± 4.50) | (9% ± 2.70) |
| CE.1.2 | CpG-free EF1α | BF5 | 125 | (91% ± 2.00) | (19% ± 0.50) |
| CE.1.3 | CpG-free EF1α | BF10 | 158 | (93% ± 1.00) | (7.5% ± 1.35) |
| UC | — | — | 164 | (90% ± 1.40) | (22% ± 2.10) |
| UC | — | — | 100 | (87% ± 1.78) | (22% ± 0.40) |
| UC | — | 86 | (91% ± 2.00) | (21% ± 2.80) |
UC: Untransfected cells used as a negative control for SCNT experiments.
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