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. 2017 Nov 14;8:1482. doi: 10.1038/s41467-017-01386-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Efficient intracellular delivery of iDR-NCs into APCs. a, b Confocal microscopy images showing that, compared with the unshrunk MFs, Alexa 488-labeled iDR-NCs were more efficiently internalized into DC2.4 cells a and RAW264.7 macrophages b. Scale bar: 50 µm. Hoechst: Hoechst 33342. LysoTracker: LysoTracker Red DND-99. Efficiently internalized iDR-NCs were co-localized with endolysosome after 3-h incubation. c Flow cytometry graphs showing efficient internalization of Alexa 488-labeled iDR-NCs by DC2.4 cells and RAW264.7 macrophages after 3 h. Red: untreated cells; blue: cells treated with iDR-NCs. d 3D-deconvolved confocal microscopy images showing one DC2.4 cell that internalized iDR-NCs into its endolysosome and cytosol after 3-h incubation. Scale bar: 1 µm. (iDR-NC concentration: 500 nM CpG equivalents)