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. 2017 Nov 14;8:1482. doi: 10.1038/s41467-017-01386-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Synergistic in vitro immunostimulation of iDR-NC nanoadjuvants. a Real-time quantitative RT-PCR results showing Stat3 silencing in DC2.4 cells treated with iDR-NCs for 48 h. Stat3 siRNA and scramble served as controls. b Flow cytometry results showing that p-STAT3 expression was down-regulated in DC2.4 cells treated with iDR-NCs for 48 h. STAT3 inhibitor (Sttatic) and Lipofectamine2000-transfected Stat3 siRNA were used as controls. Stat3 shRNA: 100 nM equivalents in a, b. c Flow cytometry results showing that iDR-NCs (100 nM CpG equivalents) elevated the expression of costimulatory factor CD80 in DC2.4 cells after 24 h. d ELISA results suggest iDR-NCs (200 nM CpG equivalents) induced DC2.4 cells to secrete significantly more TNFα, IL-6, and IL-12p40 than CpG or CpG MFs, after 24 h. Stat3 siRNA alone was used as a control. (ns: non-significant; *p < 0.05; **p < 0.01; n = 3; one-way ANOVA with Bonferroni post-hoc test). Data represented as mean ± s.e.m.

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