Fig. 6.

Efficient delivery of iDR-NCs to LN APCs in vivo. a Schematic illustration of the LN delivery study of iDR-NCs. b, c Representative 3D projection and coronal PET views b and ID% c of ⁶⁴Cu-labeled iDR-NCs in LNs one day after i.l.t. or s.c. injection. d Upper: representative flow cytometry plots showing the signals of Alexa 555-labeled iDR-NCs and CSIINFEK_(FITC)L in LN-residing B220⁺ B cells, CD11c⁺ DCs, and F4/80⁺ macrophages one day after s.c. injection at the tail base of C57BL/6 mice. Lower: pie charts showing the quantified flow cytometry results in vaccinated mice. (n = 4 in a–d; **p < 0.01; one-way ANOVA with Bonferroni post-hoc test). Data represent mean ± s.e.m. e Flow cytometry results showing elevated CD80 expression in LN DCs and macrophages one day after s.c. vaccination (vacci.) of iDR-NCs in C57BL/6 mice. Age-matched naive mice were used as controls in b, c