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. 2017 Nov 14;8:1482. doi: 10.1038/s41467-017-01386-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — iDR-NC/neoantigen elicited potent and durable neoantigen-specific T cell responses. C57BL/6 mice were s.c. immunized with CpG/shRNA^Stat3-iDR-NCs/Adpgk (2 nmol CpG equivalents, 17 μg Adpgk) on day 0 and day 14. a Schematic illustration of dextramer staining to analyze antigen-specific CD8⁺ T cells. b Representative flow cytometry and c quantification of ASMTNMELM-specific CD8⁺ T cells among live (DAPI⁻) CD8⁺ cells in PBMCs, as stained using a PE-conjugated ASMTNMELM-H-2D^b dextramer on day21. d Flow cytometry showing upregulated PD-1 expression on PBMC Adpgk⁺CD8⁺ T cells, relative to total CD8⁺ T cells, in mice vaccinated with iDR-NC/Adpgk on day21. e Upper: representative flow cytometry plots showing CD8⁺ T cell effector/effector memory/central memory phenotypes in PBMCs of naive mice and mice vaccinated with iDR-NC/Adpgk on day49; lower: quantification of the fractions of CD8 T cell populations in mice vaccinated with iDR-NC/Adpgk. iDR-NC/Adpgk induced substantial central memory (CD62L^highCD44⁺) CD8⁺ T cells, especially memory Adpgk⁺CD8⁺ T cells compared with age-matched naive mice. (***p < 0.001; n = 4; one-way ANOVA with Bonferroni post-hoc test). Data represent mean ± s.e.m.