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. 2017 Nov 13;7:15463. doi: 10.1038/s41598-017-15451-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of the MSC/β-TCP composite. (Aa,b,c) Immediately after bone marrow circulated in the SECCS, spindle cells were found on the porous β-TCP scaffold. (Ba,b,c) After 10-day in vitro culture, many spindle cells were found on the surface and internal space of β-TCP. (C) After 10-day in vitro culture, ALP staining of MSC/β-TCP composites showed red colonies, indicating ALP activity.