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. 2017 Nov 13;7:15463. doi: 10.1038/s41598-017-15451-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structure and mechanism of the screen–enrich–combine(-biomaterials) circulating system (SECCS). (A) Plastic pipe: contains a detachable filtration device, a silastic tube, and 2 3-way cocks. (B) Detachable filtration device: consists of a cap, an inner box, and an outer box. (C,D) Bone marrow injection: by adjusting the 3-way cocks, nozzle.1 opens into nozzle.2, and nozzle.4 opens into nozzle.5, which means that channel.3 is closed. Bone marrow was poured into the pipe from nozzle.2, and air was exhausted via nozzle.4. (E,F) Formation of a sealed circulatory pipe: by adjusting the 3-way cocks, nozzle.1 is connected to nozzle.5 through channel.3. Thus, a sealed pipe is assembled for bone marrow circulation.