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. 2017 Nov 13;7:15459. doi: 10.1038/s41598-017-15799-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The pumpless microfluidic device. (A) Schematic 3-D image of the PL microfluidic device, showing medium tank, tissue inlet, resistance circuit, and medium outlet. On the right, an enlarged view of the portion encompassing the tissue chamber, medium flow channel, and tissue inlet route is shown. (B) Photographs of the device. In the right, closer view of tissue chamber and resistance circuit is shown. (C) Low-lateral view of the device demonstrating the medium flow route, finally dropping down to the collecting dish.