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Line charts illustrating the differences in the phosphorylation of aged Akt (a), ERK (b) and mTOR (c) and control Akt (d), ERK (e) and mTOR (f) molecules, with cells treated with leucine and HMB over 120 min. The data is shown as mean with SD. Significance is set at P < 0.05 and was indicted versus 0 min (*) time-point. The experiment consisted of 3 repeats all in duplicate
