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. 2017 Nov 13;7:15481. doi: 10.1038/s41598-017-15588-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A,B) Circular dichroism spectra of native NfoR and its mutants in the far UV region. (C,D) Relative activity of purified NfoR and its mutants. Reaction mixtures (1 mL), containing 50 mM Tris-HCl buffer (pH 7.0), 25 μM enzyme, 0.3 mM NADH, 0.2 mM Cr (VI), and 30 μM Cu (II), were incubated for 5 min at 37 °C. Error bars indicate standard errors. (E,F) The Stern-Volmer curve of the Cu (II)-NfoR and Cu (II)-NfoR mutants at 298 K, pH 7.0.