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. 2017 Nov 13;7:15453. doi: 10.1038/s41598-017-14894-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Paladin is expressed both in the epithelial and mesenchymal compartment of the postnatal lung (4 weeks). (a–e) Combined X-gal (black) and immunofluorescence staining of Pald1 ^LacZ/LacZ mice show Pald1 LacZ expression in the vasculature, i.e. endothelial cells in capillaries (a) but to a lesser extent in endothelial cells of larger blood vessels (b) as indicated by Erg staining (endothelial cell nuclei, green). In large blood vessels LacZ expression can be detected in vascular smooth muscle cells (c, α-smooth muscle actin, red). Paladin LacZ reporter is also active in pneumocytes type II (d, SPC, green) and pneumocytes type I/II (e, cytokeratin, red). Scale bar = 20 µm.