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. 2017 Nov 13;7:15434. doi: 10.1038/s41598-017-15656-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression analysis. (A) Heat map showing gene expression assayed by RNASeq. The heat maps show gene clusters involved in glycolysis, β-oxidation, oxidative phosphorylation and the tricarboxylic acid cycle in rat hearts from 2, 6, 8, and 12 days after birth (n = 6 for both). (B) Quantitative RT-PCR analysis of genes involved in cell metabolism. Bars indicate the relative expression (2^−ΔΔct) for P1 versus P7 hearts (n = 6 for both) of Aldolase c (a glycolysis enzyme) and mitochondrial Lrrk2, Ndufv3, and Ucp2; t-test, p < 0.05.