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. 2017 Nov 13;7:15434. doi: 10.1038/s41598-017-15656-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P1 cardiomyocytes have a significant mitochondrial oxidative metabolism. (A) Time scan measurements of real-time oxygen consumption (OCR) using a Seahorse Flux Analyzer in cardiomyocyte cultures from P1 and P7 (n = 3 for both). Panels B-H show quantitative comparisons derived from experiments (mean ± SEM), such as those in panel (A,B) Basal OCR; t-test, *p < 0.05 vs. P1. (C) ATP-linked OCR; t-test, *p < 0.05 vs. P1. (D) Proton leak; t-test, *p < 0.05 vs. P1. (E) Maximal OCR; t-test, *p < 0.05 vs. P1. (F) Reserve capacity; t-test, NS. (G) Non-mitochondrial; t-test, *p < 0.05 vs. P1. (H) TMRM-stained the cell-integrated pixel area (mean ± SEM) in cardiomyocyte cultures from P1 and P7 pups (n = 3 for both); t-test, *p < 0.05 vs. P1.