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. 2017 Nov 13;7:15434. doi: 10.1038/s41598-017-15656-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cardiac cell proliferation does not alter tissue metabolic profiles. (A) Bars indicate oxygen consumption (mean ± SEM) with glucose (5 mM) measured using Oroboros O2k high-resolution respirometry in P1, P7, and sham resected neonatal hearts 5 days (+5) after the procedure (S1 + 5d, n = 8 and R1 + 5d, n = 3; S7 + 5d, n = 7 and R7 + 5d, n = 4); two-way ANOVA, *p < 0.05 vs. 1d + 5. (B) Lactate production (mean ± SEM) measured in the oxygen consumption conditioned buffer from P1 and P7 sham and resected neonatal hearts five days after the procedure (S1 + 5d, n = 8 and R1 + 5d, n = 3; S7 + 5d, n = 7 and R7 + 5d, n = 4); two-way ANOVA, NS. (C) O₂/CO₂ rate (mean ± SEM) calculated from O₂ and CO₂ concentration in the oxygen consumption conditioned buffer from P1 and P7 sham and resected neonatal hearts 5 days after the procedure (S1 + 5d, n = 9 and R1 + 5d, n = 3; S7 + 5d, n = 7 and R7 + 5d, n = 4); two-way ANOVA, NS.