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. 2017 Nov 13;7:15444. doi: 10.1038/s41598-017-15670-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased nuclear 8-Oxo-dG and loss of the mismatch repair components, MSH2 and MSH6, in airway epithelial cells in IPF lungs. (A–L) Normal and IPF lung biopsies were stained with anti 8-Oxo-dG (A and D, G and J, respectively), anti MSH2 (B and E, H and K, respectively) and anti MSH6 (C and F, I and L, respectively). Shown above are representative images taken at 50x (top) and 200x (bottom) magnification from 3 normal and 10 IPF lungs. (M–P) Normal and IPF lung explants were mechanically dissociated and the resulting cellular suspensions were fixed, permeabilized and stained with anti-EpCAM and anti-MSH2 antibodies. Shown are representative flow cytometric dot plots for EpCAM⁺ (M) and MSH2⁺ cells within the EpCAM⁺ population (O) epithelial cells stained normal (top) and IPF (bottom) and the average percentage of cells showing EpCAM (N) and EpCAM and MSH2 (P) from 4 normal and 10–11 IPF lung explants. (Q–T) Shown are representative histograms depicting MSH2 and BRCA1 expression (Q and S, respectively) and GMFI (R and T, respectively) from 4 normal and 9 IPF lung explant derived EpCAM⁺ cells.