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. 2017 Nov 13;7:15397. doi: 10.1038/s41598-017-14987-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(Near)infrared polymethine dyes for in vivo use. (A) Excitation and (B) Emission spectra of DY-654 and DY-780 in PBS, 0.1% DMSO. λ_em or λ_ex in (A) and (B) refer to the wavelength at which the dyes were excited to measure emission spectrum or the wavelength at which emission was measured while measuring excitation spectrum. (C) Absorption spectra of unmixed and equimolar mixed dyes. (D) Specific excitation and emission wavelengths allow the simultaneous detection of both dyes in whole blood. (E) Toxicity (LDH-assay) after 24 h on primary murine hepatocytes for DY-654 and DY-780 was assessed revealing no cellular toxicity. LDH release is expressed in % to completely lysed cells (High) by Triton-X solution. DY-780 was incubated in 0.005% DMSO, therefore a vehicle-control (DMSO) was included (F) The alamar blue assay revealed no change in cell viability of primary murine hepatocytes after incubation with DY-654, DY-780 or vehicle (medium or DMSO) for 24 h (E,F) Hepatocytes from 10 (LDH-Assay) or 6 (Alamar Blue Assay) different animals were used for the experiment.