Fig. 6.

Possible p53 sensor applications. a Graphical illustration of the sensor’s use in large-scale drug or RNAi/CRISPR screens to uncover p53 stabilizers and/or drugs that restore WT p53 function in mutant cells. Important steps are highlighted by arrows. b Functional p53 profiling in primary tumor samples. A model to determine p53 activity in primary cancer cells is presented. c Clinical application of the sensor in AML patients with altered p53. An illustration to couple the sensor to oncolytic viruses and its application in a combination with radiotherapy/chemotherapy before bone marrow transplantation is presented. Red and white dots illustrate leukemic p53 mutant cells and healthy donor cells after transplantation in the patient, respectively. Arrows highlight important steps