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. 2017 Nov 13;7:15389. doi: 10.1038/s41598-017-15258-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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μ-opioid agonist inhibition of forskolin-stimulated cAMP production assay. The concentration-response curves for morphine and buprenorphine determined in both the absence and presence (1, 3, 10, 30, 100, 300 nM) of the irreversible antagonist β-funaltrexamine are represented jointly in the same graph. The concentrations at which the curves for each agonist cross with each other are marked. Results were obtained in at least three independent experiments. In each experiment, data points were obtained in quadruplicates.