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. 2017 Nov 13;7:15425. doi: 10.1038/s41598-017-15846-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HGF upregulates miR-7 in MCF-10A cells. (A) Cells were treated with HGF for 24 hours, after which the expression level of miR-7 was measured by qPCR. (B) HGF expression was downregulated with siRNA (siHGF) and the expression level of miR-7 was measured by qPCR 24 hours after transfection. siNC, control siRNA. (C and D) miR-7 was overexpressed using mimic miR or downregulated using an inhibitor miR. Twenty-four hours after treatment, the expression level of HGF was measured by qPCR. The expression levels of HGF and miR were normalized to GAPDH and U6 RNA, respectively. All experiments were performed in triplicate and indicated as mean ± SE. siNC, siRNA negative control; miNC, microRNA negative control.