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. 2017 Oct 5;8:42–51. doi: 10.1016/j.omtm.2017.09.008

Figure 4.

Figure 4

Uptake of rhIDU into MPS I Human Fibroblasts and Murine Macrophages

(A–C) Intracellular IDU activity per million cells in the absence of immune serum (A), in the presence of inhibiting immune serum (IS) (anti-rhIDU antibody titer 752 OD U/μL) (B), or in the presence of partially inhibiting immune serum (PIS) (titer 450 OD U/μL) (C), with and without mannose 6-phosphate (M6P) or an Fc receptor blocking agent (FcR block). For all panels, uptake into human fibroblasts is shown as filled bars and uptake into murine macrophages is shown as open bars. (A–C) were run together as a single experiment (replicated five times). Error bars represent SD. *p < 0.05 versus human fibroblasts. (D) Intracellular rhIDU uptake into murine macrophages in the presence of no serum (RhIDU), nonimmune serum (Ser), PIS, or purified rabbit anti-rhIDU IgG antibodies (IgG) with or without M6P. IDU activity is expressed as a percent of uptake into murine macrophages in the absence of any factors. Error bars represent SD. *p < 0.05 versus rhIDU alone.