Figure 2.

Comparison of Single and Double Vascular Injections of pDSYF in BlAJ/NRG Mice in a 4-Week Experiment

(A) Timeline of the experiment; all mice received a first injection of plasmid pDYSF at day 0, whereas half of the mice also received a second injection of pDYSF at day 14. Luciferase live imaging was performed 2 and 28 days after injections. All mice were harvested at day 28. (B) Representative luciferase live images of mice 28 days after single (left) or double (right) doses of pDYSF. (C) Bioluminescence radiance values were graphed for control (HBSS alone) and single and double doses of pDYSF. Although double dose values trended higher, the difference in levels observed between the single and double dose groups was not statistically significant. (D) Western blot images of protein extracted from the four hind limb muscle groups from two representative legs after injection with either HBSS or a double dose of pDYSF. NRG represents the wild-type level of dysferlin, whereas NRG/BlAJ represents the null level of dysferlin in BlAJ/NRG mice. (E) Western blot band quantification of all the data showed an upward trend in dysferfin expression in the double dose groups that was not statistically significant. The dotted line represents 100% of wild-type levels of dysferlin. Q, quadriceps; H, hamstring; T, tibialis anterior; G, gastrocnemius. Data are mean ± SEM, with n = 3 to 4.