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. 2017 Oct 24;7:123–131. doi: 10.1016/j.omtm.2017.10.005

Figure 3.

Figure 3

Vascular Injection of pDYSF or pDYSF-FST in BlAJ/NRG Mice in a 12-Week Experiment

(A) Timeline of the experiment; all mouse legs received a single vascular injection of either HBSS alone, pDYSF, or pDYSF-FST at day 0, and hind limb muscles were collected at 12 weeks. Luciferase live imaging was performed at 2 days, 6 weeks, and 12 weeks after injection. (B) Representative luciferase images of the same mouse at 2 days, 6 weeks, and 12 weeks after injection with pDYSF-FST. (C) Similar levels of bioluminescence were observed in mice injected with pDSYF and pDYSF-FST. These levels were persistent over the time course of the experiment. (D) Representative western blot images of three mouse legs injected with pDYSF-FST; the H, Q, and G muscle groups were analyzed. NRG represents the wild-type level of dysferlin, whereas NRG/BlAJ represents the null level of dysferlin in BlAJ/NRG mice. Lane L is the protein size ladder. (E) Western blot band quantification showed no significant difference in dysferfin expression between the two plasmids FST. The dotted line represents 100% of wild-type levels of dysferlin present in NRG mice. Q, quadriceps; H, hamstring; G, gastrocnemius. Data are mean ± SEM, with n = 4–13.