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Design of Two PD-1 TALEN Targeting the First Exon of PDCD1 Locus
(A) Schematic representation of the PDCD1 (PD-1) genomic sequence. Black boxes represent exons, and triangles represent the approximate TALEN-binding sites. (B and C) Schematic representations of the loci and T3v1 (B) and T1 (C) PD-1 TALEN used to knock out PDCD1 in primary T cells.
