Figure 5.

miR-3174 Promotes CDDP Resistance in GC Cells

(A) The relative expression of miR-3174 in CDDP-resistant MKN45 and BGC823 cells and their parental CDDP-sensitive cells was detected with RT-PCR. Cell viability of MKN45 (B) or BGC823 (D) cells with or without miR-3174 reconstitution and (C) MKN45CDDP or (E) BGC823CDDP cells with or without miR-3174 inhibition was measured with CCK-8 assays after exposure of cells to different concentrations of CDDP for 48 hr. (F–I) The IC₅₀ value of each group was determined. (J and K) Colony formation assays were performed in CDDP-sensitive (J) or -resistant (K) cells to detect cell long-term viability after indicated concentrations of CDDP stimulation for 4 hr. Graph represents mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.