Fig. 3.

Effect of LA on oxidative stress in the brains of P301S mice. (A) ROS production was detected in the brains of each group by the DCF-DA method. (B) SOD activity levels in the brains of each group. (C-F) Western blot and quantification analysis of SOD1, GPx4, and xCT with β-actin as a loading control. (G) Detection of calcium content by atomic absorption spectrometry. (H) Immunofluorescence with anti-GPx4 antibody and MitoTracker Green in the hippocampus and cortex. All results are presented as the mean ± SEM (n = 7). *p < 0.05, **p < 0.01.