FIG. 2.

Quantification of astrocytes present in mESC cultures. (A) Representative images showing A2B5, Aqp-4, and merged staining in protoplasmic and fibrous cultures 6 days after differentiation. Blue represents Hoechst nuclear stain. Scale bar: 100 μm. (B) Representation flow cytometry graphs of secondary controls and samples stained for Aqp-4 and A2B5 for both fibrous and protoplasmic cultures. Each graph represents 10,000 cells based on an FSC and SSC gate. Quadrant gates drawn based on single stained samples and secondary controls. Numbers represent the percent of cell events within each quadrant (C) average percentage of cells staining for A2B5, Aqp-4, and both markers in fibrous and protoplasmic cultures. n = 5, error bars: standard error, **P < 0.01. (D, E) Average percent of total cells staining β-tubulin⁺ (neurons) (D) or S100⁺ (E). N = 4, error bars: 95% confidence interval. **P < 0.01. Aqp-4, aquaporin 4; FSC, forward scatter; SSC, side scatter. Color images available online at www.liebertpub.com/scd