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. 2017 Nov 15;26(22):1597–1611. doi: 10.1089/scd.2017.0121

FIG. 3.

FIG. 3.

Protoplasmic astrocyte-derived substrates are permissive to motoneuron growth and neurite extension. (A–G) Representative images of motoneurons on different astrocyte-derived substrates after 48 h in culture. (A–C) Fibrous substrates, (D–F) protoplasmic substrates. (A, D) Live astrocyte substrate, (B, E) freeze-lysed astrocyte substrate, (C, F) decellularized astrocyte substrate. (G) Gelatin control shows no clear neurites. Scale bar: 100 μm (H) quantification of neurite extension from motoneurons cultured for 48 h on different astrocyte substrates. Error bars: standard error, n = 19–52. *P < 0.05, **P < 0.01, ***P < 0.001. Dark bars = protoplasmic substrate, light bars = fibrous substrate.