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. 2017 Nov 15;26(22):1597–1611. doi: 10.1089/scd.2017.0121

FIG. 4.

FIG. 4.

Protoplasmic astrocyte-derived substrates are more permissive to V2a interneuron growth than fibrous astrocyte-derived substrates. (A–E) Representative images of V2a interneurons after 72 h in culture on different astrocyte-derived substrates. (A, B) Fibrous substrates, (D, E) Protoplasmic substrates, (A, D) freeze-lysed astrocyte substrate, (B, E) decellularized astrocyte substrate. (C) Unmodified gelatin control. Scale bar: 100 μm. (F) Quantification of neurite extension from V2a interneurons cultured for 3 days on different astrocyte substrates. Error bars: Standard error, n = 40–56, ***P < 0.001. Dark bars = protoplasmic substrate, light bars = fibrous substrate.