FIG. 7.

Viral knockdown modifies motoneuron growth on astrocyte ECMs. (A–E) Aqp-4 (red) and A2B5 (green) staining of infected astrocytes. (A–C) Protoplasmic astrocytes infected with nontargeting (A), laminin α5 targeting (B), or laminin γ1 targeting (C) virus. (D, E) Fibrous astrocytes infected with nontargeting (D), or spondin-1 targeting (E) virus. Scale bar: 100 μm. (F) Spondin-1 knockdown in fibrous ECM leads to a significant increase in motoneuron growth. n = 39–58. (G) Laminin α5 or laminin γ1 knockdown decreases motoneuron growth on protoplasmic ECM. n = 44–104. Error bars: standard error; *P < 0.05; **P < 0.01; ***P < 0.001. Color images available online at www.liebertpub.com/scd